The Pilgrims will host City's development team, who are the reigning WSL Development League champions, at The Jakemans Stadium on Sunday August 19 (kick-off 2.00pm).
United community manager Nick Reeson said: "We have been speaking to Manchester City for a while now about a possible fixture and I am delighted they have agreed to travel to Boston to play our Ladies team.
"Having one of the world’s biggest clubs in women's football come and agree to play you, it's something that has really excited everyone at the club and everyone is looking forward to it.
"Both Kat Nilsen and Kevin Murphy have been very helpful at Manchester City and we would like to thank them for supporting us."
United Ladies had a successful season in 2017-2018, winning the treble and gaining promotion to the East Midlands Women's Regional League for the first time and the visit of City will coincide with a celebration to promote women's and girls football in Lincolnshire and the presentation of last season's league title and medals to the Ladies team.
Tickets will be priced at £5 for Adults (over 16), and £3 for Under 16s and Concessions.
If you are interested in being either a match or matchball sponsor for this game, please contact Ceri Jackson on (01205) 364406 or via e-mail.
Updated 14:37 - 12 Jul 2018 by Ceri Jackson