Paul Cox's Pilgrims will entertain the Sandgrounders (kick-off 3.00pm) to begin their 13th consecutive campaign at Step 2.
A whole host of new signings could make their competitive debuts for the Pilgrims following an extensive recruitment drive.
Cox will check on the fitness of loanees Sam Long (thigh) and Jean Leroy Belehouan (concussion) ahead of kick-off. United's only definite absentee is striker Jordan Burrow (ankle).
Southport, led by Liam Watson, fell out of play-off contention late on last season. Their attack is spearheaded by ex-Pilgrim Jordan Archer.
Last season's corresponding fixture ended 1-1, with Luke Shiels rescuing a point at the death.
LIVE COVERAGE
BBC Radio Lincolnshire online full-match commentary, courtesy of Rob Makepeace and Craig Singleton, is available.
TICKETS
The match is NOT all-ticket – but ticket purchases are recommended. Click here to buy a ticket.
TRANSPORT
Click here for a full guide on transport to and from the Jakemans Community Stadium - with car parking reserved exclusively for seasonal pass holders and club guests. A limited number of car parking spaces are on sale for this fixture (here).
ELLENDERS
Ellenders will be open from 11.00am.
HOSPITALITY
Doors for Club 85 and the executive boxes will open at 12.30pm.