United head to The Wong (kick-off 7.45pm) looking to complete a quickfire double over Horncastle following a 1-0 home win earlier in the month.
Former Horncastle favourites Tony Edwards and Richard Jackson both feature in the Boston squad, while youngsters Lewis Sturman, James Reed, Jake Beauchamp and Charley Sanders are included too.
Kevin Austin, who is not involved on Wednesday, scored the only goal of the game when the sides last met.
Horncastle were also 2-1 victors over United at The Wong in a Challenge Cup tie earlier in the campaign.
United (from): Hewitt, Beauchamp, Bishop, Dawson, Edwards, Eustace, Ihama, Jackson, Lovett, Ogden, Rawdon, Reed, Sanders, Sturman, A Thompson, M Thompson, Whitaker.