Scunthorpe United will play at Step 2 for the first time after relegation from the National League.
Elsewhere, promoted sides South Shields, Warrington Town, Tamworth, Rushall Olympic and Bishop's Stortford will all join National League North too.
National League North - 2023-2024
Alfreton Town
Banbury United
Bishop's Stortford
Blyth Spartans
Boston United
Brackley Town
Buxton
Chester FC
Chorley
Curzon Ashton
Darlington
Farsley Celtic
Gloucester City
Hereford FC
King's Lynn Town
Peterborough Sports
Rushall Olympic
Scarborough Athletic
Scunthorpe United
South Shields
Southport
Spennymoor Town
Tamworth
Warrington Town
Fixtures are expected in the first week of July - with the new season kicking off on Saturday August 5.